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Comparative study of rates of rates of transpiration in the upper and lower surfaces of leaf, using four-leaf experiment

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  Practical Notebook Biology Standard XII Biology  Answeres Practical Notebook Standard XII Class-12th science Click here download in PDF B. DEMONSTRATION EXPERIMENTS 1. Comparative study of rates of transpiration in the upper and lower surfaces of the leaf, using four-leaf experiment.  7 Loss of excess water in the form of vapor through the aerial parts of the plant is called transpiration.  Aim: To study the comparative rate of transpiration in the upper and lower surfaces of a leaf by conducting four-leaf experiments.   Requirements: -  Two stands, thread, four leaves of Calotropis / banyan tree, Vaseline (petroleum jelly), etc.  Fig.  Comparison of transpiration, a demonstration by four-leaf experiment Apply Vaseline to leaf surface in the following manner,  1. Leaf A: - Petroleum jelly is applied on both surfaces  Figure: Procedure:  The leaves selected should be healthy preferably of the same size and showing growth.  Tie the different leaves of the same plant with thread in suc

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