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To identify common disease causing organisms like Plasmodium, Entamoeba, Ascaris and Ringworm with the help of permanent slides and or specimens. Comment on symptoms of diseases that they cause

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Practical Notebook Biology Standard XII To identify common disease-causing organisms like Plasmodium, Entamoeba, Ascaris, and Ringworm with the help of permanent slides and or specimens. Comment on symptoms of diseases that they cause Biology  Answeres Practical Notebook Standard XII Class-12th science Click here download in PDF  Plasmodium (Disease - Malaria):  Plasmodium vivax is an intracellular, blood parasite in human vertebrates which causes malaria.   The life history of Plasmodium is completed in two hosts, partly in the definitive host, the man, and partly in an intermediate host, the female Anopheles mosquito.   When an infected female anopheles mosquito bites a man, sporozoites are introduced into the bloodstream from where they reach liver cells and multiply to form merozoite.   After a few cycles in the liver, the merozoite enters the RBC and feeds on the contents of the RBC.   After 2-8 schizogenous changes in the main bloodstream, the merozoite assumes a different shape

To examine the presence or absence of Barr body in the given sample

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  Practical Notebook Biology Standard XII Biology  Answered. Practical Notebook Standard XII Class-12th science Click here download in PDF 12. To examine the presence or absence of Barr body in the given sample.   Alm:  To observe Barr bodies in squamous epithelial cells obtained from the inner lining of the check (Buccal smear).  Requirements: Cotton buds, slide saline solution, 70% alcohol, Giemsa / Methylene blue stain compound microscope, etc  Procedure:  Clean the mouth by rinsing and gargling with water, several times.   Take a sterile cotton bud and in normal saline solution   Rub it on the inner lining of the cheek several times.   Smear the bud on a clean slide and allow it to dry.   Place the slide in a Petri dish.  Layer it with a fixative (70% alcohol).    Allow it to stand for a few minutes.    Drain off the extra fixative.    Stain the smear with Giemsa / Methylene Blue and wait for 10 minutes.    With the help of a dropper gently wash off the excess stain with water.  

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