Observe the prepared slide of blood smear to identify different types of blood cells Project Download in Pdf

 Practical Notebook Biology Standard XII

Observe the prepared slide of a blood smear to identify different types of blood cells Project Download in Pdf

Chapter 14

Biology 

Answeres

Practical Notebook
Standard XII

Class-12th

science

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14. Observe the prepared slide of the blood smear to identify different types of blood cells.

 Aim:- To identify and observe the types of blood artis in freshly prepared blood smear.

Requirements:-

Blood sample slides, Lentoon's stain, compound microscope.

Procedure 

A Hoxed or used to say or only the types of Mood cells but also the To be able to see the different types of cells in the poravites, if any, in the Mood of Pla Mood, the hood Mr bod in other words layer on the slide This spread of Mood on a slide is called Smear 

To observe all the cells present in the blood sample under the microscope, they must be stained appropriately.  This process is called Staining Various staining like Leithmon's stain or Wright's stain can be used for storming the Mood cells 

Preparation of the Smar:-

1. Take two clean and dry slides 
2.  Place a drop of blood towards one end of a slide this slide) 
3. Hold this slide having the drop of blood 
4. Take the other slide one hand the spreader side ) and keep it inclined on the first slide such that is just touching the edge of the drop of blood 
5. Hold the second slide in this position till the drop has spread evenly along its edge.  
6. Gently move the spreader slide once ever the base slide to forms a thin layer of blood, called Blood Smear.  The smear should cover approximately half the slide 
7. Leave the smear for air drying 

The staining of the Blond smear completely 

1. With the help of a dropper put s drugs of Lochmon's sham on the slide to cover the smear completely.
2. Keep the stain on the slide for about 5 minutes for its action.  Do not allow the stain to dry.  Add a few drops of distilled water, if the stain dries 
3. Drain off the excess stain and wash the slide under gentle running tup water till the excess stain is removed 
4.  Allow the slide to dry at room temperature.  Observe it first under low power and under high power of a compound microscope.  
5. Observe and identify the different types of blood cells.  

Observation

1. The main cellular component of mammalian blood is the erythrocytes or RBCs.  They are circular biconcave cells without a nucleus.  The cells contain a large amount of hemoglobin 
2. A number of leukocytes or WBCs are also found.  They are generally irregular in shape and show prominent nuclei.  The leucocytes are divided into two main types Le Granulocytes having granular cytoplasm and Agranulocytes ( W.BCs with a clear non-granular cytoplasm ).  Depending upon the shape of the nucleus and the nature of granules, the granulocytes are further divided into acidophils, basophils, and neutrophils.  The Agranulocytes are divided into two sub-types lymphocytes and monocytes 
3. Thrombocytes ( platelets ) are also present in the blood.  They are spindle-shaped cells.  However, normally these are not visible in the casual preparations.