To study wing shape and eye colour in Drosophila

 Practical Notebook Biology Standard XII

Biology 

Answeres

Practical Notebook
Standard XII

Class-12th

science

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11. To study wing shape and eye color in Drosophila.  

Aim: 

To study the genetic variation of traits in Drosophila melanogaster.  Requirements: Glass jar, petri dish, banana pieces, muslin cloth, bell jor, slide, coverslips grated series of school, fine, DP%, etc.

Procedure L Preparation of culture beds: 

1. Peel the ripe banana fruit.  Discard the skin and mash the fruit into a soupy pulp.  
2. Take a clean sterilized glass jar or Petri dish and place the banana pulp in it.  
3. Add a few grains of yeast.  
4. Keep a small blotting paper to absorb excess moisture from the pulp and also to provide a substratum for the pupation of the larvae.  
5. Many culture beds can be prepared by the above method for the different varieties of a fruit fly.  II The culturing of Drosophila 
6. The pulp in the culture beds attracts the Drosophila.  Cover the culture beds with a bell jar after the Drosophila has reached the pulp.  They complete their life cycle within 48 hours.  
7. Capture the Drosophila and fix it into 70% alcohol.  
8. Process the Drosophila flies through 70%, 90% and 100% graded series of ethyl alcohol for minutes each.  
9. Place Drosophila in Xylene for 5 min. and clear it properly.  
10. Mount it on a glass slide with DPX and observe it under the microscope.  Observation and Result: Culture 1 Culture 2 Eye Color Red (wild) Black (mutant) Pattern of Wing Normal Vestigial No.  of segments in a leg Nine number of segments Less number