Isolation of DNA from Given Sample
Practical Notebook Biology Standard XII
Biology
Answeres
Practical Notebook
Standard XII
Class-12th
science
9. Isolation of DNA from Given Sample
Aim:
To extract and isolate DNA from a fruit sample.
Requirements: -
Fleshy berry fruits like banana, Zizyphus, grapes, etc, can be used. Fleshy fruits of banana, liquid soap, distilled water, salt (NaCl), 1000 ml ice-cold isopropyl alcohol i.e. IPA (Chill the alcohol (IPA) by placing the test tube in a beaker containing ice cubes and some water] measuring spoons, glass stirring rod, test tubes, glass beakers, plastic cups, strainer or coffee filter, funnel, etc. Principle: All plants' DNA extraction protocols comprise the basic steps of disruption of a cell wall, cell membrane, and nuclear membrane to release the DNA into solution followed by precipitation of DNA and ensuring removal of the contaminating biomolecules. A figure is separated by physical means such as grinding, to form a mash
- The cells in a fruit sample blend.
- Put 2 cups of distilled water and one banana blend in it. Then pour the mixture into the beaker
- Mix 01 teaspoon of liquid soap with a teaspoon of salt in a plastic zip-lock bag or cup Add 02 tablespoons of distilled water and stir gently until the soap and salt have dissolved the mixture for 10-15 minutes by using a glass rod.
- Add 02 tablespoons of banana mash mixture to the cup containing salt and soap solution. Stir
- After 15-20 minutes filter the fruit mixture through a fine sieve or coffee filter.
- Take a test tube of chilled alcohol (IPA). Add filtrate to it with the help of a dropper, ver slowly.
- Place the test - tube undisturbed for 5-6 minutes.
- A layer of alcohol floating on the top of the fruit mixture is now clearly visible.
- The white material becomes visible as a precipitate that marks the isolated DNA.
- It can be spooled out with the help of a hook or bent paperclip or glass rod. Slowly draw them out of the solution.
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